2018
Phytochemical screening and antibacterial effect of Zizyphus lotus methanolic extract against oral pathogenic bacteria.
La revue : Journal of Fundamental and Applied Sciences
Domaine : sciences de la nature et de la vie
Mots Clés : zizyphus lotus- methanolic extract-
Auteur : T.Aicha, B. Hamida, M.Boumediene
Issn : 1112-9867 Eissn : vol : 10, Num : (2), pp : 252-266
Résume :

The aim of this in vitro study was to assess the antibacterial effect of the methanolic extract obtained from the roots of Zizyphus lotus(communally Sedra) collected in Mascara on analysis which revealing 433,65μg EGA / mg dm ofcollected from the oral cavity (10 students) against bacteria responsible of oral pathologies, isolated from students aged from 20 to 28 years. Chemical qualitative analysis of this extract revealed the presence of polyphenols, flavonoids and tannins confirmed by a quantitative analysis polyphenols, 247,87μg EQ / mg dm of flavonoids and 167.05 μg ECT/ mg dm of tannins.microbiological analysis of samples bacterial groups (streptococci, staphylococci, lactobacilli and enterobacteria).Extract of Zizyphus lotus tested on agar medium and microdilution method showed an important inhibition diameter of 40 mm respectively towards Lactobacillus sp.

2015
Effect of Mentha viridis extracts on pathogenic bacteria adhesion
La revue : Journal of Natural Remedies JNR
Domaine : sciences de la nature et de la vie
Mots Clés : Mentha viridis- adhesion- pathogenic bacteria- extract
Auteur : T.Aicha,M; Catherine ,B.Mohamed, M.Boumediene
Issn : 2320-3358 Eissn : vol : 15, Num : 1, pp : 12-22
Résume :

Micro-organisms do not always exist in planctonic forms (single cells or small groups). To survive, especially in limiting media, they may adhere to inert or living surfaces. This enables them to multiply within a community protected by an extracellular matrix, thus forming a biofilm which protects them from antimicrobials especially antibiotics. In this paper, the aim of this study was to investigate autoaggregation, co-aggragation, surface hydrophobicity and exopolysaccharides production of different pathogenic bacteria treated with methanolic, hydro-methanolic and aqueous extract of Mentha viridis collected in El Bayadh (South West of Algeria). The qualitative analysis revealed the presence of polyphenols and flavonoids, which were confirmed-by-quantitative-analysis. All extracts showed an antibacterial activity (antiadhesiveness).The percentage of autoaggregation, coaggregation decreased in presence of extract comparing to control. E.coli was hydrophobic while the others hydrophilic. The amount of exopolysaccharides (EPS) produces by bacteria decreased considerably with all extracts comparatively to control (without extracts). Keywords: antiadhesiveness activity, extracts, Mentha viridis, multi-resistant bacteria, polyphenols.

2015
Antioxidant activity and exopolysaccharide-expression of pathogenic bacteria treated with Mentha viridis extracts
La revue : Journal of Chemical and Pharmaceutical Research
Domaine : sciences de la nature et de la vie
Mots Clés : exopolysaccharides-antioxydant activity- pathogenic bacteria- mentha viridis
Auteur : T.Aicha,M.Catherine B.Mohamed, M.Boumediene
Issn : 0975-7384 Eissn : vol : 7, Num : 6, pp : 90-95
Résume :

Natural extracts contain a variety of phenolic compounds which have an antioxidant and antibacterial capacity;they can successfully replace antibiotics that show their inefficiencies against multi-resistant bacteria. In this context, this study was intended as a contribution to the understanding and evaluation of the bioactive potential of methanolic, hydro-methanolic and aqueous extracts of Mentha viridis. The qualitative analysis revealed the presence of polyphenols and flavonoids, which were confirmed-by-quantitative-analysis. The extracts of Mentha viridis expressed an important anti-production activity of the Exopolysaccharides by all pathogens strains. The amount of exopolysaccharides (EPS) produces by bacteria decreased considerably with all extracts comparatively to control (without extracts). The extracts showed strong properties to trap molecules of free radical DPPH with an inhibition power ranging from 45 to 85%. Key words: antibacterial, antioxidant, extracts, Mentha viridis, multi-resistant bacteria

2012
In vitro and in vivo antimicrobial activity of Algerian Hoggar Salvadora persica L. extracts against Microbial strains from children’s oral cavity
La revue : Journal of Ethnopharmacology 144 (2012) 57–66
Domaine : sciences de la nature et de la vie
Mots Clés : Salvadora persica- enfants scolarisés- antimicrobial activity- in vitro- in vivo
Auteur : T.Aicha,M.Catherine, A. Abdelkader ,M.Boumediene
Issn : 0378-8741 Eissn : vol : 144, Num : , pp : 57-66
Résume :

Ethnopharmacological relevance: Salvadora persica L. (miswak) is traditionally used to ensure oral hygiene Muslim people in developing countries where it is growing. The antibacterial properties of Salvadora persica L. originating from various geographic areas have already been reported. However, they have never been tested for samples originating from Hoggar, where extreme weather conditions could lead to different properties for this Salvadora persica L. ecotype. Aim of the study: To assess the in vitro and in vivo antimicrobial activities of methanolic extract of Algerian Hoggar Salvadora persica L. (miswak) on some isolated and identified strains from the oral cavity of school children aged from 6 to 12 with (n¼20) and without (n¼20) caries. Materials and methods: After a qualitative and quantitative analysis of dental plaque samples from the selected children, the effect of methanolic extract of Hoggar miswak against oral bacterial and fungal strains isolated from the oral cavity of children with caries was tested by both agar disc diffusion and microdilution methods. The stability and physicochemical parameters of Hoggar Salvadora persica L. mouthwash were also assessed compared. The in vivo antimicrobial effect of Hoggar miswak rinse on dental plaque samples was also tested over a week. Results: Four bacterial genera (Staphylococcus, Streptococcus, Escherichia and Lactobacillus) were commonly identified in all subjects at different colonization levels. A statistically significant difference in colonization levels between the two groups of children was recorded. Hoggar miswak extract more significantly inhibited the growth of Gram negative bacteria from the dental plaque than Gram positive ones. Hoggar Salvadora persica L. mouthwash was stable at 4 1C and 25 1C over the period of conservation (one week) while a temperature of 40 1C induced variations in the physicochemical parameters and considered not suitable for preservation. The in vivo study revealed a significant reduction in bacteria of the oral cavity using miswak mouthwash as compared to placebo. Conclusion: Hoggar miswak extract displayed a strong antimicrobial effect both in vitro and in vivo. Its use as a mouthrinse could therefore be recommended as a preventive measure to preserve from tooth decay.